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ea0022p55 | Adrenal | ECE2010

Influence of various clinical variables and storage conditions on urinary cortisol levels: gas chromatography–mass spectrometry (GC–MS) versus immunoassay

Deutschbein Timo , Broecker-Preuss Martina , Hartmann Michaela , Wudy Stefan , Althoff Ricarda , Mann Klaus , Petersenn Stephan

Introduction: Measurement of urinary cortisol is often used to assess disease activity in patients with suspected or proven hypercortisolism. However, specific reference ranges are lacking for some of the newer assays. This study analyzed upper limits of normal (ULN, mean+2S.D.) for two analytical procedures (GCMS, ECLIA) in relation to various independent variables. Besides, the influence of different storage conditions was investigated (by ECLIA).<p ...

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ea0016p44 | Adrenal | ECE2008

Quantitative real time RT-PCR of CYP11B2 (aldosterone synthase) to confirm the diagnosis of aldosterone-producing adenomas

Ueberberg Bianca , Althoff Ricarda , Deutschbein Timo , Unger Nicole , Hinrichs Jakob , Walz Martin K , Schmid Kurt W , Petersenn Stephan

Background: The diagnosis of hyperaldosteronism is hindered by the absence of a well-defined gold-standard. CYP11B2 (aldosterone synthase) belongs to the steroid-metabolizing enzymes catalyzing the last step of the aldosterone synthesis. To establish a molecular marker for aldosterone-producing adenomas, we compared the expression in various adrenal tumors.Methods: Tissue from 20 aldosterone-producing adenomas (APA), 12 hyperplasias associated with hyper...

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Endocrine Abstracts

Influence of various clinical variables and storage conditions on urinary cortisol levels: gas chromatography–mass spectrometry (GC–MS) versus immunoassay

Quantitative real time RT-PCR of CYP11B2 (aldosterone synthase) to confirm the diagnosis of aldosterone-producing adenomas

BiosciAbstracts